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更新時間：2022-01-29
Easy and fast detection of mycoplasma contamination in cell culturesWith over 40 years of experience in developing mycoplasma solutions for the scientific community, InvivoGen's MycoStrip™ offers a …
With over 40 years of experience in developing mycoplasma solutions for the scientific community, InvivoGen's MycoStrip™ offers a new method for the detection of mycoplasma in cell culture, allowing you to combat the contamination.
MycoStrip™ is a simple test that requires no special lab equipment, and provides clear results, with no false positives. Furthermore, with results in less than an hour, MycoStrip™ allows you to swiftly start treating the contaminated cell culture.
Detection of cell culture contaminating mycoplasma by MycoStrip™ is based on isothermal PCR. Simply prepare your sample and add our proprietary Reaction Mix to target and amplify the 16S rRNA gene for the most commonly found mycoplasma species in cell culture. Results are clearly visualized on an immunochromatographic strip within 5 minutes.
Easy to perform, and unlike most other mycoplasma detection assays, no special lab equipment is required.
? Rapid
Performed in 1 hour, with less than 15 minutes of hands-on time.
? Clear
Fast and easy-to-interpret results that appear within 2-5 minutes, as 1 or 2 bands on the strip.
One band - negative for mycoplasma
Two bands - positive for mycoplasma
? Specific
MycoStrip™ has been specifically designed to detect the Mycoplasma and Acholeplasma species that most commonly contaminate cell culture. These include the six species that account for 95% of all contaminations: M. orale, M. hyorhinis, M. arginini, M. fermentans, M. hominis, and A. laidlawii. Importantly, because the MycoStrip™ reaction mix detects unique regions of DNA there is no cross?reactivity with other bacterial, fungal or mammalian DNA.
? Sensitive
MycoStrip™ is able to detect as low as 10-102 CFU/ml and thus, can be used to detect mycoplasma contamination before it significantly affects experimental results, which typically occurs at ~107 CFU/ml [1].
? Also available without cassettes
The larger unit size (100 strips) contains no cassette, offering a more economical and eco-friendly version of MyscoStrip™
References:
1. Drexler, H.G. & Uphoff, C.C. 2002. Mycoplasma contamination of cell cultures: Incidence, sources, effects, detection, elimination, prevention. Cytotechnology 39, 75-90.
Click to enlarge
| With cassette | Without cassette | |||
| Contents | 10 Tests | 20 Tests | 50 Tests | 100 Tests |
| • Reaction Mix • Reaction Buffer • Migration Buffer • Positive Control • Detection strips | 1 vial 1 vial 2 vials 1 vial 10 | 2 vials 2 vials 3 vials 1 vial 20 | 5 vials 4 vials 1 bottle 1 vial 50 | 10 vials 8 vials 2 bottles 2 vials 100 |
MycoStrip™ is shipped at room temperature.
Store all components (including the detection strips) at -20°C.
If properly stored, all components are stable for 12 months.
Mycoplasma is a major problem in cell culture, with various sources of contamination such as lab operators, dirty water baths/incubators, and biological reagents. Due to their small size, mycoplasma is undetectable by visual inspection, they pass through standard filtration, and are resistant to a large number of antibiotics. There are many reasons to regularly test your cell culture for mycoplasma and these include:
Loss of precious samples
Serious impact on data reliability and reproducibility
Testing is required by most journals for publication
Loss of time and money
More information on mycoplasma in cell culture
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