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      技術文章您現在的位置:首頁 > 技術文章 > GeneTex熱銷抗體 — GAPDH抗體原理/介紹

      GeneTex熱銷抗體 — GAPDH抗體原理/介紹

      更新時間:2024-12-13   點擊次數:274次

      GAPDH抗體識別甘油醛-3-磷酸脫氫酶(GAPDH)蛋白,相基因的產物催化碳水化合物代謝中一個重要的能量產生步驟,即在無機磷酸鹽和煙酰胺腺嘌呤二核苷酸(NAD)存在的情況下,對甘油醛-3-磷酸進行可逆的氧化磷酸化。GeneTex熱銷抗體 —— GAPDH抗體(克隆號GT239)助理您的科研實驗!

       

      產品詳情

      產品名稱GAPDH antibody [GT239]

      別名:glyceraldehyde-3-phosphate dehydrogenase , G3PD , GAPD , HEL-S-162eP

      反應種屬:Candida albicans, Chinese Hamster, Dog, Drosophila, E. coli, Hamster, Human, Monkey, Mouse, Pig, Rat, Yeast, Zebrafish

      宿主來源:Mouse

      實驗應用:EMSA, ICC/IF, IHC-P, WB

      同種型:IgG2b

      免疫原:Recombinant protein encompassing a sequence within the center region of human GAPDH. The exact sequence is proprietary.

      克隆性:Monoclonal

      克隆號:GT239

      純化方式:Affinity purified by Protein G.

      偶聯:Unconjugated

      產品濃度:1 mg/ml (Please refer to the vial label for the specific concentration.)

      保存溫度:Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4oC. For long-term storage, aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles.

      運輸溫度:4°C

      預期分子量:36 kDa

      產品形式:Liquid

      存儲溶液:PBS, no preservative.

       

      驗證數據

      GTX627408.png

       WB Image

      GAPDH antibody [GT239] detects GAPDH   protein by western blot analysis.

      A. 30 μg yeast whole cell   lysate/extract

      10% SDS-PAGE

      GAPDH antibody [GT239] (GTX627408)   dilution: 1:10000

      The HRP-conjugated anti-mouse IgG   antibody (GTX213111-01) was used to detect the primary antibody.

      GTX627408(1).png

      ICC/IF:

      GAPDH antibody [GT239] detects GAPDH   protein at cytoplasm by immunofluorescent analysis.

      Sample: HeLa cells were fixed in   ice-cold MeOH for 5 min.

      Green: GAPDH stained by GAPDH antibody   [GT239] (GTX627408) diluted at 1:500.

      Blue: Hoechst 33342 staining.

      GTX627408(2).png

      IHC-P:

      GAPDH antibody [GT239] detects GAPDH   protein at cytoplasm by immunohistochemical analysis.

      Sample: Paraffin-embedded mouse kidney.

      GAPDH stained by GAPDH antibody [GT239]   (GTX627408) diluted at 1:200.

      Antigen Retrieval: Citrate buffer, pH   6.0, 15 min

       

      產品部分引用文獻

      1.S Shivaprasad et al. Viruses 2024; 16 (5) : 725 CRISPR Screen Reveals PACT as a Pro-Viral Factor for Dengue Viral Replication.

      2.X Chen et al. Nature 2024; 628 (8009) : 818-825 Antisense oligonucleotide therapeutic approach for Timothy syndrome.

      3.CP Chen et al. Heliyon 2024; 10 (7) : 29055 Synergistic effects of the combination of trametinib and alpelisib in anaplastic thyroid cancer with BRAF and PI3KCA co-mutations.

      4.YL Chen et al. Viruses 2024; 16 : 523 Novel Anti-Viral Properties of the Herbal Extract of Davallia mariesii against Influenza A Virus.

       

      訂購詳情

      貨號

      產品名稱

      規格

      GTX627408-S

      GAPDH antibody

      25 μl

      GTX627408

      100 μl

       

      如需了解更多詳細信息或相關產品,請聯系GeneTex中國授權代理商-欣博盛生物 

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