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ENZO熱銷產品——TOTAL-NUCLEAR-ID® green/red nucleolar/nuclear detection kit核仁／細胞核檢測試劑盒（綠色／紅色）
ENZO的TOTAL-NUCLEAR-ID® green/red nucleolar/nuclear detection kit包含兩種專有染料，適用于同時對細胞核和核仁進行活細胞染色。這些染料可以檢驗由細胞周期和核糖體發生等生物過程引起的細胞內分布、運輸和定位的核仁動態變化。該試劑盒與大多數熒光檢測系統兼容，包括常規和共聚焦熒光顯微鏡，以及高內涵篩選（HCS）平臺。TOTAL-NUCLEAR-ID® green/red nucleolar/nuclear detection kit專門設計用于觀察活細胞中的核仁和細胞核，潛在應用包括監測受損核糖體的生物生成、抑制轉錄、細胞周期動力學和細胞應激，以及核仁蛋白的分布、運輸和動力學，病毒蛋白的分布，并可能有助于識別癌癥細胞。
產品特點
● 同時對活細胞中的核仁和細胞核染色
● 高耐光漂白性和抗淬滅，即使在長時間觀察后也能確保強烈一致的熒光信號
● 已驗證在活細胞成像應用中的實用性，對核仁擾動劑的治療有適當的反應性
● 生產嚴格，以控制和消除非特異性結果
實例分析
● NUCLEOLAR-ID®綠色檢測試劑（A）和NUCLEOR-ID®紅色檢測試劑（B）的吸收和發射光譜
● 用NUCLEOLAR-ID®綠色檢測試劑染色的細胞在核仁內顯示出最大的熒光信號，在整個細胞核和細胞質中顯示出微弱的熒光（A）。當用低劑量放線菌素D處理細胞時，觀察到核仁染色丟失（B）。與較弱的細胞質染色相比，使用NUCLEOLAR-ID®紅色檢測試劑進行反染色有助于突出核仁。
● 用TOTAL-NUCLEAR-ID® Green/Red Nucleolar/ Nuclear Detection Kit對U2OS活細胞進行染色，通過熒光顯微鏡觀察細胞核（紅色）和核仁（黃綠色）的染色結果。作為一種RNA染料，NUCLEOLAR-ID®綠色染料也顯示出一些細胞質染色，但NUCLEOR-ID®紅色染料有助于在高亮顯示的紅色細胞核熒光信號范圍內，將核仁明確識別為綠色熒光信號。
● U2OS細胞中染色為紅色的細胞核和染色為綠色的核仁的3D重建
產品詳情：
| 產品名稱 | TOTAL-NUCLEAR-ID® green/red nucleolar/nuclear detection kit |
| 產品貨號 | ENZ-51006-500 |
| 產品規格 | 500 assays |
| 質量控制 | A sample from each lot of TOTAL-NUCLEAR-ID® Green/ Red Nucleolus/Nucleus Detection Kit is used to stain HeLa cells using the procedures described in the user manual. Cells stained with NUCLEOLAR-ID® Green Detection Reagent show maximal fluorescence signal within the nucleoli, with only faint fluorescence throughout the nucleus and cytoplasm. NUCLEAR-ID® Red Detection Reagent maximally stains the DNA in the cell nucleus. Cells induced by actinomycin D show reduction of nucleolar signal. |
| 應用 | Fluorescence microscopy, Fluorescent detection For visualizing nucleoli and nuclei simultaneously in living cells. |
| 短期儲存 | -20°C |
| 長期儲存 | -80°C |
| 組分 | NUCLEOLAR-ID® Green Detection Reagent, 50 µL NUCLEAR-ID® Red Detection Reagent, 50 µL Actinomycin D Control, 125 µg 10X Assay Buffer, 15 mL |
| 產品說明 | The TOTAL-NUCLEAR-ID® green/red nucleolar/nuclear detection kit is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. |
部分產品文獻引用
1. Guanidinylated bioresponsive poly(amido amine)s designed for intranuclear gene delivery: J. Yu, et al.; Int. J. Nanomedicine 11, 4011 (2016), Application(s): Confocal microscopy using MCF7 cells
2. Dose-dependent role of the cohesin complex in normal and malignant hematopoiesis: A.D. Viny, et al.; J. Exp. Med. 212, 1819 (2015), Application(s): Confocal microscopy
3. Small-molecule multi-targeted kinase inhibitor RGB-286638 triggers P53-dependent and -independent anti-multiple myeloma activity through inhibition of transcriptional CDKs: D. Cirstea, et al.; Leukemia 27, 2366 (2013), Application(s): Nucleolar segregation analysis by immunofluorescence
4. Mismatch in mechanical and adhesive properties induces pulsating cancer cell migration in epithelial monolayer: M.H. Lee, et al.; Biophys. J. 102, 2731 (2012), Application(s): Detection of nuclei and nucleoli in breast cell lines using fluorescence microscopy
5. AFM stiffness nanotomography of normal, metaplastic and dysplastic human esophageal cells: A. Fuhrmann, et al.; Phys. Biol. 8, 015007 (2011), Application(s): Fluorescence lifetime imaging of normal and dysplasic esophageal cells
6. Strategies of actin reorganisation in plant cells: A.P. Smertenko, et al.; J. Cell Sci. 123, 3019 (2010), Application(s): Staining of plant cells
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