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      技術(shù)文章您現(xiàn)在的位置:首頁(yè) > 技術(shù)文章 > Enzo Life Sciences欣博盛生物:NUCLEAR-ID®系列染料

      Enzo Life Sciences欣博盛生物:NUCLEAR-ID®系列染料

      更新時(shí)間:2023-07-27   點(diǎn)擊次數(shù):572次

      Enzo Life Sciences公司的NUCLEAR-ID® Blue DNA stain (GFP-CERTIFIED®)NUCLEAR-ID ® Red DNA Stain是細(xì)胞滲透性染料,適用于活細(xì)胞的細(xì)胞核染色。這系列染料為通過(guò)流式細(xì)胞術(shù)研究細(xì)胞周期進(jìn)程的誘導(dǎo)和抑制提供了一種簡(jiǎn)便方法。該試劑在活細(xì)胞研究中的潛在應(yīng)用包括確定細(xì)胞DNA含量和細(xì)胞周期分布、檢測(cè)生長(zhǎng)模式的變化、監(jiān)測(cè)細(xì)胞凋亡以及評(píng)估腫瘤細(xì)胞行為和抑制基因機(jī)制。

       

      NUCLEAR-ID® Red DNA stain

       

      產(chǎn)品特點(diǎn)

      ● 遠(yuǎn)紅色熒光特異性DNA染料

      ● 穩(wěn)定且純度高

      ● 可分析活細(xì)胞、可滲透細(xì)胞和固定細(xì)胞

      ● 無(wú)光漂白作用

      ● 無(wú)需RNase處理

      ● GFPFITC兼容

      ● 不需要紫外線激光源激發(fā)

      ● 經(jīng)驗(yàn)證適用于各種細(xì)胞密度

      ● 快速且易于使

       

      實(shí)例分析

      37°C下用終濃度為4000x2000xNUCLEAR-ID ® Red DNA Stain同等µM濃度的競(jìng)爭(zhēng)品牌染料對(duì)細(xì)胞進(jìn)行染色,并在染色后輕輕洗滌。15分鐘24小時(shí)對(duì)細(xì)胞成像,結(jié)果顯示NUCLEAR-ID ® Red DNA Stain在更低的濃度就能顯現(xiàn)dsDNA


      ENZO熱銷產(chǎn)品——NUCLEAR-ID®系列染料

       

      產(chǎn)品詳情:

      產(chǎn)品名稱

      NUCLEAR-ID® Red DNA stain

      產(chǎn)品貨號(hào)

      ENZ-52406

      產(chǎn)品規(guī)格

      200µl

      最大激發(fā)波長(zhǎng)

      566nm

      最大發(fā)射波長(zhǎng)

      650nm

      純度

      ≥93%HPLC

      質(zhì)量控制

      1.Absorption peak of NUCLEAR-ID® Red dye: λmax = 566 ± 4 nm

      2.% purity of NUCLEAR-ID® Red dye by HPLC: ≥93%

      應(yīng)用

      Flow Cytometry, Fluorescent detection

      短期儲(chǔ)存

      -20°C

      長(zhǎng)期儲(chǔ)存

      -80°C

      產(chǎn)品說(shuō)明

      The NUCLEAR-ID® Red DNA Stain is a member of the CELLESTIAL® product line, reagents and assay 

      kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis 

      applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such 

      as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.

       

       

      部分產(chǎn)品文獻(xiàn)引用

      1. CD200R1L is a functional evolutionary conserved activating receptor in human neutrophils: M.I.P. Ramos, et al.; J. Leukoc. Biol. 111, 367 (2022)

      2. FOXM1 network in association with TREM1 suppression regulates NET formation in diabetic foot ulcers: A.P. Sawaya, et al.; EMBO Rep. 23, e54558 (2022)

      3. Multi-dimensional-double-spiral (MDDS) inertial microfluidic platform for sperm isolation directly from the raw semen sample: H. Jeon, et al.; Sci. Rep. 12, 4212 (2022)

      4. DYRK1A negatively regulates CDK5-SOX2 pathway and self-renewal of glioblastoma stem cells: B. Chen, et al.; Int. J. Mol. Sci. 22, 4011 (2021)

      5. Global phosphoproteomics reveals DYRK1A regulates CDK1 activity in glioblastoma cells: A. Recasens, et al.; Cell Death Discov. 7, 81 (2021)

      6. Hydrogen Peroxide Is Crucial for NLRP3 Inflammasome-Mediated IL-1β Production and Cell Death in Pneumococcal Infections of Bronchial Epithelial Cells; Journal of Innate Immunity: S. Surabhi, et al.; J. Innate Immun. 10, 1159 (2021)

      7. Licochalcone A inhibits hypoxia-inducible factor-1α accumulation by suppressing mitochondrial respiration in hypoxic cancer cells: M.K. Park, et al.; Biomed. Pharmacother. 133, 111082 (2021)

       

      NUCLEAR-ID® Blue DNA stain (GFP-CERTIFIED®)

       

      產(chǎn)品特點(diǎn)

      ● 藍(lán)色熒光特異性DNA染料

      ● 穩(wěn)定且純度高

      ● 可分析活細(xì)胞、可滲透細(xì)胞和固定細(xì)胞

      ● 無(wú)光漂白作用

      ● 無(wú)需RNase處理

      ● GFP和紅色染料兼容

      ● 經(jīng)驗(yàn)證適用于各種細(xì)胞密度

      ● 快速易用

       

      產(chǎn)品詳情:

      產(chǎn)品名稱

      NUCLEAR-ID® Blue DNA stain (GFP-CERTIFIED®)

      產(chǎn)品貨號(hào)

      ENZ-CHM103-0200

      產(chǎn)品規(guī)格

      200µl

      最大激發(fā)波長(zhǎng)

      350nm

      最大發(fā)射波長(zhǎng)

      461nm

      純度

      ≥95% (HPLC)

      外觀

      Frozen liquid

      應(yīng)用

      Flow Cytometry, Fluorescent detection

      短期儲(chǔ)存

      -20°C, Protect from light. Avoid freeze/thaw cycles

      長(zhǎng)期儲(chǔ)存

      -80°C, Protect from light. Avoid freeze/thaw cycles

       


       ENZO熱銷產(chǎn)品——NUCLEAR-ID®系列染料

      詳情請(qǐng)聯(lián)系 ENZO 金牌代理商-欣博盛生物科技

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